Figure 1

Detection of Trypanosoma cruzi parasites in samples of the deceased child. A and B, sections of cerebral cortex stained with hematoxylin and eosin, and C, brain stem stained with Giemsa stain. The magnification is shown in the lower right corner; C is digitally magnified. Arrows indicate: in A, an inflammatory nodule, in B, parasites in the cytoplasm, and in C the nucleus (N) and kinetoplast (K) of one of the amastigotes. D, Results of Tcz1-Tcz2 PCR (lanes 1 to 5) and Ln-PCR (lanes 6 to 10) from serum. Lanes 1 and 6, serum; lanes 2, 5, 7 and 10, negative controls; lanes 3 and 8, T. cruzi positive control; lanes 4 and 9, Leishmania positive control; M, molecular weight markers (PCR marker 50 – 2000 bp).