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Table 1 Detection of anti-Trypanosoma cruzi and anti-Leishmania antibodies in the family members of the deceased child.

From: Fatal congenital Chagas' disease in a non-endemic area: a case report

 

T. cruzi tests

Leishmania tests

 

In-housea ELISA

In-houseb IFAT

rk39-ELISAa

In-houseb IFAT

Commercialb IFAT

Mother

2.09

> 1/320

0.04

N

1/1280

Father

0.05

N

0.01

N

N

Brother 1

2.10

1/320

0.07

± 1/80

1/320

Brother 2

0.04

N

0.01

N

N

Deceased child

1.89

> 1/320

0.03

N

nd

  1. NOTE. - aResults are expressed as absorbances. The cut-off in the T. cruzi ELISA test was 0.5, and 0.2 in the rk39 ELISA test.
  2. bThe IFAT results show the serum dilutions in which fluorescent parasites were observed. The cut-off for T. cruzi was 1/40, and 1/80 for Leishmania. N = negative, ± = parasites observed with and without fluorescence, nd = not done.