Table 1 Results of light microscopy and immunohistochemistry

Toluidin-blue and HE-staining

Focally accentuated chronic myopathy with endo-, peri- and epimyseal fibrosis, reduced fiber thickness and augmentation of endomysial connective tissue

Trichrome-staining

Focal subsarcolemmal fuchsinophily, centralised cores

PAS-staining

Regular content of muscle-glycogene

Elastica-von-gieson and oil-red-staining

No amyloid

NADH-TR-enzymeimmunohistochemistry

Normal forming of groups of type I and II muscles, predominance of type I muscle fibers

Immunohistochemistry for myofiber-ATPasis (3 diff. pH's)

Mainly type-I-muscle-fibers/also type-II muscle-fibers had central encymatic defects

Succinatedehydrogenasis and cytochrome-c-oxidasis activity

Normal

Lead-citrate and uranyl-acetate cuts

Muscle-fibers with subsarcolemmal deposited triglycerides and dilatated vacuoles, internal structure partially loosened

Antibodies anti-dystrophine-epitopes 1, 2 and 3 and anti-ubiquitine, merosin, desmine and alpha-sarcoglycane

Regular colouring

Antibodies anti LCS, CD 3 and CD 20

Normal number of mononuclear cells