Table 1 Antibodies used and immunohistochemical results
From: Hepatocellular carcinoma with characteristic mucin production: a case report
Specificity | Clones | Dilution | Pretreatment | Manufacturer | Results | |||
|---|---|---|---|---|---|---|---|---|
Tumor | Cirrhotic nodule | |||||||
Trabeculae | Glands | Tubule | Hepatic cord | |||||
CK7 | OV-TL 12/30 | 1:200 | pepsin | Dako, Glostrup, Denmark | +* | + | ++ | + |
CK19 | RCK108 | 1:200 | pepsin | Dako | ++ | ++ | + | - |
CK20 | Ks20.8 | 1:200 | pepsin | Dako | ++ | ++ | - | - |
AE1 | AE1 | 2× (predilute) | pepsin | Zymed, San-Francisco, CA | ++ | ++ | + | - |
AE3 | AE3 | 1:200 | pepsin | Zymed | - | - | - | - |
CEA | polyclonal | 1:200 | pepsin | Zymed | + | + | - | - |
EMA | E29 | 1:400 | pepsin | Dako | +/- | + | + | - |
AFP | ZSA06 | 1:200 | pepsin | Zymed | + | - | - | - |
HepPar1 | OCH1E5 | 1:150 | microwave | Dako | + | - | +/- | - |
c-kit | 1:150 | microwave | Dako | - | - | - | - | |
CD34 | QBEnd 10 | 1:400 | pepsin | Dako | - | - | - | - |
Chromogranin-A | DAK-A3 | 1:200 | microwave | Dako | - | - | - | - |