Hereditary bleeding disorder, factor ix deficiency in females: a case series

Cases Journal

Table 1

Case No.	Screening test			Diagnostic test correction study			Factor IX assay	Fibrinogen* (normal range 200-400 mg/dl)
				Mix of Factor IX def. plasma and test plasma (1.1)	Mix of Factor VIII def. plasma and test plasma (1.1)	Mix of Factor IX def. plasma and test plasma (1.1)
	PT (sec)	APTT (sec)	TT (sec)	APTT (sec)	APTT (sec)	APTT (sec)
Case 1	C:14	C:24	C:10	26	28	68	42%	288 mg/dl
	T:14	T:52	T:11
Case 2	C:14	C:28	C:10	28	28	82	37%	310 mg/dl
	T:14	T:68	T:11
Case 3	C:14	C:24	C:10	25	30	140	26%	325 mg/dl
	T:14	T:85	T:12
Remarks		Raised APTT indicates intrinsic pathway defect			Correction of APTT with normal plasma and FVIII def. plasma and no correction with FIX def. plasma indicates absence of inhibitor & def. of factor IX.

Abbreviations: APTT, activated partial thromboplastin time; def, deficient/deficiency; Mix, mixture; PT, prothrombin time; TT, thrombin time. The method used for PT, APTT, TT, and fibrinogen was manual technique using commercial reagent (from Diagnostic Stago) as described by Pitney & Brozovic [5].
* The method used for factor IX assay, was manual technique using commercial factor VIII and IX deficient plasma (from Diagnostic Stago) and as described by Pitney & Brozovic [5] (Semi quantitative assay).